Gemella bacteraemia characterised by 16S ribosomal RNA gene sequencing

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Gemella bacteraemia characterised by 16S ribosomal RNA gene sequencing.

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BACKGROUND Globicatella are streptococcus-like organisms that have been rarely isolated from clinical specimens. Their epidemiology and clinical significance remain largely unknown. AIMS To describe two cases of Globicatella bacteraemia identified by 16S ribosomal RNA (rRNA) gene sequencing. METHODS Two unidentified streptococcus-like bacteria isolated from blood cultures of patients were s...

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Identification by 16S ribosomal RNA gene sequencing of Arcobacter butzleri bacteraemia in a patient with acute gangrenous appendicitis.

AIMS To identify a strain of Gram negative facultative anaerobic curved bacillus, concomitantly isolated with Escherichia coli and Streptococcus milleri, from the blood culture of a 69 year old woman with acute gangrenous appendicitis. The literature on arcobacter bacteraemia and arcobacter infections associated with appendicitis was reviewed. METHODS The isolate was phenotypically investigat...

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Next-generation sequencing of 16S ribosomal RNA gene amplicons.

One of the major questions in microbial ecology is "who is there?" This question can be answered using various tools, but one of the long-lasting gold standards is to sequence 16S ribosomal RNA (rRNA) gene amplicons generated by domain-level PCR reactions amplifying from genomic DNA. Traditionally, this was performed by cloning and Sanger (capillary electrophoresis) sequencing of PCR amplicons....

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ژورنال

عنوان ژورنال: Journal of Clinical Pathology

سال: 2003

ISSN: 0021-9746

DOI: 10.1136/jcp.56.9.690